Studies on mannanase of Streptomyces. Part III. Purification and some properties of mannanase from Streptomyces sp.
نویسندگان
چکیده
منابع مشابه
Optimization of Mannanase Production from Streptomyces Sp. Pg-08-03 in Submerged Fermentation
Streptomyces sp. PG-08-3 was isolated from the desert of Rajasthan (India). The organism produced mannanase (15 Umg protein) in the presence of 0.5% guar gum as a sole carbon source in minimal media by submerged fermentation (SmF). Enzyme production was enhanced by 7.3-fold when 0.5% soyabean meal and 0.25% of leucine were added to the minimal media. Increasing the guar gum concentration in the...
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β-Mannanase SACTE_2347 from cellulolytic Streptomyces sp. SirexAA-E is abundantly secreted into the culture medium during growth on cellulosic materials. The enzyme is composed of domains from the glycoside hydrolase family 5 (GH5), fibronectin type-III (Fn3), and carbohydrate binding module family 2 (CBM2). After secretion, the enzyme is proteolyzed into three different, catalytically active v...
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An alkaliphilic Bacillus isolate (strain JAMB-750) was found to exoproduce a novel alkaline mannanase (AmA). AmA was purified to homogeneity (about 98% pure) as judged by SDS-polyacrylamide electrophoresis. The molecular mass was approximately 130 kDa. The hydrolysis patterns of various substrates indicated that the enzyme was an endo-type enzyme. The optimal temperature and pH for activity wer...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1984
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.48.2189